Fig 1: OV-REC8 and apcin promotes MCF-7 cell apoptosis. (A) Effect of apcin + OV-REC8 on MCF-7 cell apoptosis was measured by TUNEL staining assay. (B) Statistical analysis of apoptotic rate. (C) Western blot analysis of Bcl2, cleaved PARP, PARP, cleaved caspase-3 and caspase-3 expression in MCF-7 cells. Data are presented as the mean ± SD and are representative of three independent experiments. **P<0.01, ***P<0.001 vs. Control; ΔΔΔP<0.001 vs. apcin + OV-NC. CDC20, cell division cycle 20; NC, negative control; OV, overexpression; PARP, poly(ADP-ribose) polymerase.
Fig 2: Overexpression of CDC20 reverses the inhibitory effect of REC8 overexpression on proliferation, migration and invasion of MCF-7 cells. (A) Western blot and (B) Reverse transcription-quantitative PCR analysis of CDC20 expression in MCF-7 cells. Cell (C) viability and (D) proliferation were measured by Cell Counting Kit-8 and (E) colony formation assay, respectively. Effect of OV-CDC20 on MCF-7 cell (F and G) migration and (H) invasion was determined by wound healing and (I) Transwell assay. (J) Western blot analysis of MMP2 and MMP9 expression in MCF-7 cells. Data are presented as the mean ± SD and are representative of three independent experiments. *P<0.05, **P<0.01, ***P<0.001 vs. OV-REC8. OV, overexpression; MMP, matrix metalloproteinase; NC, negative control; OD, optical density; CDC20, cell division cycle 20.
Fig 3: Knockdown of CDC20 in hepatocellular carcinoma cells. Protein expression levels of CDC20 in (A) Hep3B and (B) Huh-7 cells, as determined by western blotting. Protein expression of CDC20 in (C) Hep3B and (D) Huh-7 cells, as determined by immunofluorescence staining (magnification, ×200). *P<0.05. CDC20, cell division cycle 20; NC, negative control; Si, small interfering RNA.
Fig 4: REC8 negatively regulates CDC20. (A and B) RT-PCR and western blot analysis of CDC20 expression in various breast cancer cell lines and (C and D) MCF-7 cells transfected with OV-NC and OV-REC8. Data are presented as mean ± SD. Results are representative of three independent experiments. **P<0.01, ***P<0.001 vs. OV-NC. CDC20, cell division cycle 20.
Fig 5: CDC20 expression in HCC tissues and HCC cell lines. (A) mRNA expression levels of CDC20 in HCC and adjacent noncancerous tissues were analyzed by Gene Expression Profiling Interactive Analysis. (B) mRNA expression levels of CDC20 in HCC and adjacent noncancerous tissues were analyzed in 71 clinical HCC samples. (C) Protein expression levels of CDC20 in HCC cells. *P<0.05, **P<0.01 vs. THLE2 or as indicated. CDC20, cell division cycle 20; HCC, hepatocellular carcinoma; N, normal tissue; T, tumor tissue; LIHC, liver hepatocellular carcinoma.
Supplier Page from Abcam for Anti-Cdc20 antibody [CDC20/1102]